Ovine iPSC

Ovine iPSC

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Ovine iPSC morphology in 2D culture

Ovine induced pluripotent stem cells (iPSCs)

Our ovine induced pluripotent stem cells (iPSC) are derived and cultured feeder-free in serum-free media. They can differentiate into multiple cell types. Characterisation includes qPCR, flow cytometry, karyotyping and sterility tests. The cells differentiate into adipocytes and protocols for scale-up and further differentiation are under development.

Alkaline phosphatase stain of ovine iPSCs

Product information

  • Cell type category: induced pluripotent stem cells (iPSC)
  • Species: Ovis aries (common name: sheep)
  • Tissue of origin: Neural
  • Re-programming method: In-house non-integrative methods
  • Fill size: Standard vials (approx. 1 x 10^6 cells)
  • Shipping method: Dry ice
  • Storage: -150°C or liquid nitrogen
Adipocytes derived from ovine iPSCs in 2D culture (stained with Oil Red O)

Culture details

  • Cell culture format: Adherent and suspension
  • Doubling time: <32 hours
  • Feeder-free derivation and culture
  • Derived and cultured in serum-free media
  • Differentiation protocols available
  • Shown to differentiate into: Adipocytes
Adipocytes derived from ovine iPSCs in 3D culture as aggregates (confocal image)

QC data available

  • Sterility/mycoplasma testing
  • Prion testing
  • Alkaline phosphatase (AP) staining
  • Karyotyping by G-Banding
  • qPCR:
    • Pluripotency: GBX2, LIN28A, SALL4, DNMT3B
    • Embryoid Body (EB) differentiation: GATA6, KRT8, VIM
    • Adipocyte (fat) differentiation: PPARy, FABP4, CEBPa
  • Flow cytometry:
    • Pluripotency: OCT4, SOX2, LIN28A
  • Oil Red O staining for adipocyte (fat) differentiation

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