Ovine ESC

Ovine ESC

We offer the most advance cell line solution for cultivated meat

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Ovine ESC morphology in 2D culture

Ovine embryo-derived stem cells (ESCs)

Our ovine embryo-derived stem cells (ESCs) are derived and cultured feeder-free in serum-free media. Characterisation includes qPCR, flow cytometry, karyotyping and sterility tests. Protocols for scale-up and differentiation are under development.

Alkaline phosphatase stain of ovine ESCs

Product information

  • Cell type category: Embryo-derived stem cells (ESC)
  • Species: Ovis aries (common name: sheep)
  • Breed: Texel X Mixed crossbreed
  • Tissue of origin: Embryo
  • Fill size: Standard vials (approx. 1 x 10^6 cells)
  • Shipping method: Dry ice
  • Storage: -150°C or liquid nitrogen
Ovine ESC doubling times during long-term stability study

Culture details

  • Cell culture format: Adherent (suspension not yet tested)
  • Doubling time: <24 hours
  • Feeder-free derivation and culture
  • Derived and cultured in serum-free media
  • Shown to differentiate into: Fat and muscle differentiation not yet tested
Ovine ESC flow cytometry analysis for pluripotency markers

QC data available

  • Sterility/mycoplasma testing
  • Alkaline phosphatase (AP) staining
  • Karyotyping by G-Banding
  • qPCR:
    • Pluripotency: LIN28A, SALL4, POU5F1, DMNT3B
    • Embryoid Body (EB) differentiation: KRT8, MYF5, VIM, DCN, GATA6
  • Flow cytometry:
    • Pluripotency: OCT4, SOX2, LIN28A, SSEA4

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