Bovine ESC

Bovine ESC

We offer the most advance cell line solution for cultivated meat

Bovine ESC morphology in 2D culture

Bovine embryo-derived stem cells (ESCs)

Our bovine embryo-derived stem cells (ESCs) are sourced from cattle with full traceability and veterinary certification. Derived from IVF and in-vivo fertilised flushed embryos, the cells are cultured feeder-free in serum-free media, with <24-hour doubling time in 2D and 3D culture. They differentiate into a wide range of cells including MPs, MSCs, adipocytes, myocytes, and fibroblasts. Characterisation includes qPCR, flow cytometry, karyotyping, RNAseq and sterility tests.

Alkaline phosphatase stain of bovine ESCs

Product information

  • Cell type category: Embryo-derived stem cells (ESC)
  • Species: Bos taurus (common name: cow)
  • Breeds: Angus x Simmental crossbreed & Holstein
  • Tissue of origin: Embryo
  • Traceability: Full traceability
  • Vetinary records: Derived from veterinary-certified animals
  • Fertilisation method: Cell lines derived from in-vitro fertilised (IVF) embryos and in-vivo fertilised (flushed) embryos are available
  • Fill size: Standard vials (1 x 10^6 cells) available
  • Shipping method: Dry ice
  • Storage: -150°C or liquid nitrogen
Adipocytes derived from bovine ESCs, as aggregates in 3D culture

Culture details

  • Cell culture format: Adherent and suspension
  • Doubling time: <24 hours in both 2D and 3D culture
  • Feeder-free culture
  • Derived and cultured in serum-free media
  • Differentiation and scale-up protocols available
  • Shown to differentiate into: Mesodermal progenitors (MPs), mesenchymal stem cells (MSCs), adipocytes, myocytes, fibroblasts
Bovine ESC flow cytometry analysis for pluripotency markers

QC data available

  • Sterility/mycoplasma testing
  • Alkaline phosphatase (AP) staining
  • Karyotyping by G-Banding
  • qPCR:
    • Pluripotency: OCT4, NANOG, SOX2
    • Trilineage differentiation: PAX6, NES, SOX17, CXCR4, MYF5 and ACTA2
    • MSC differentiation: CD44, CD73
    • Adipocyte (fat) differentiation: FABP4, CEBalpha
  • Flow cytometry:
    • Pluripotency: SSEA4, OCT4, NANOG, SOX2
    • MSC differentiation: CD44, CD73
  • Oil Red O staining for adipocyte (fat) differentiation

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